The Basics of Transgenic and Agrobacterium
Keywords:
Agrobacterium, Ti-Plasmid, transgenics, vir genes, transgenic plantAbstract
Transgenesis is an extremely powerful tool to analyses and manipulate the genetics of mice and other animals. As defined above, a transgene is a segment of experimentally introduced DNA, carried in a host animal's genome. A transgene may be designed to encode a new gene product in the transgenic animal, or it may be introduced at its place of insertion in order to alter or disrupt a host gene. In many cases, for example, a transgene will both disrupt an endogenous gene while expressing a new gene product. Thus transgenesis applications take advantage of its ability to induce genetic alterations in both loss-of-function and gain-of-function. The basics of the transgenics have been explained in this review. In addition, how Agrobacterium tumefaciens has been described as being used in transgenic processReferences
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