Complexation With Chromium Makes an Nonutilizable Amino Acid Lysine into a Utilizable Form in S. cerevisiae (NCIM 3558) Through Changes in Intracellular Compartmentation of the Amino Acid

Authors

  • K. S. Karthikeyan Post-Doctoral fellow, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad
  • K. Sivarama Sastry Post-Doctoral fellow, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad

DOI:

https://doi.org/10.37628/ijcbcp.v3i2.228

Abstract

The present study deals with the complete reversion of the utilizability of an otherwise nonutilizable L-amino acid herein L-lysine in Saccharomyces cerevisiae NCIM 3558 on complexation with chromium sulfate hexahydrate. It is observed for the first time that following complexation of all the inhibitory effect of L-lysine on yeast is completely reversed, and the detailed mechanism is discussed and the mechanism of action is dealt in detail.

References

K. Schwartz, W. Mertz. Chromium (III) and the glucose tolerance factor, Arch Biochem Biophy. 1952; 85: 292–5p.

E.W. Toepfer, W. Mertz, M.M. Polansky, E.E. Roginski, nd W.R. Wolf. Preparation of chromium containing material of GTF activity from brewer’s yeast extracts and by synthesis, J Agric Food Chem. 1977; 25: 162–6p

G. Galuszka, M.C. Golonka, A. Szelag, J. Starosta, A. Wojciechouska. Synthetic models for the Glucose tolerance factor: spectroscopic characterization and toxicity studies of monomeric and dimeric Cr (III) species, Polyhedron. 1998; 17: 3785–94p

X. Yang, K. Palanichamy, A.C. Ontko, M.N.A. Rao, C.X. Fang. A newly synthetic chromium complex-chromium (phenylalanine)3 improves insulin responsiveness and reduces whole body glucose tolerance, FEBS Lett. 2005; 579: 1458–64p.

K.S. Karthikeyan, H. Polasa, G. Reddy. Effects of amino-acid chromium complexes on yeast metabolism, Proc A.P. Acad Sci. 2008; 34: 178–83p.

S.B. Shuttleworth, R.L. Sykes. The mode of co-ordination of amino acids with cationic chromium in acid aqueous solution I. Spectrophtometic studies, J Am Leather Chem Assoc. 1959; 54: 259–68p.

V. Uma, H. Polasa. Rapid production of ethanol by newly isolated S. cerevisiae of palm vine, J Microbiol Biotechnol. 1988; 3: 70–4p.

T.G. Watson. Amino acid pool composition of S. cerevisiae as a function of growth rate and amino acid nitrogen source, J Gen Microbiol. 1976; 96: 263–8p.

H. Vogel, Y. Shimura. Lysine estimation, Methods Enzymol. 1971; 17B: 238–9p.

E. Albers, C. Larsson, G. Liden, C. Nikalsson, L. Gustafsson. Influence of Nitrogen source on S. cerevisiae anaerobic growth and product formation, Appl Environ Biol. 1996; 62: 3187–95p.

T. Sato, Y. Oshumi, Y. Anraku. Substrate Specificites of active transport system for amino acids in vacuolar membrane vesicles of S. cerevisiae, J Biol Chem. 1984; 251: 11505–8pz.

Published

2017-12-21

Issue

Section

Articles