Isolation of Human Umbilical Vein Endothelial Cells (HUVECs) from Umbilical Cord and Characterization of HUVECs in Terms of Cell Surface Markers via Flow Cytometry
DOI:
https://doi.org/10.37628/ijmb.v1i1.50Keywords:
endothelial cells-culture-characterization, flow cytometry, HUVECsAbstract
This work was carried out to investigate the isolation, culture and characterization of human umbilical vein endothelial cells (HUVECs) in vitro by the help of flow cytometry, and also to improve the cell yield and growth of these cells in culture. The endothelial cells were isolated by collagenase digestion and were cultured in tissue culture flasks using Dulbecco's modified Eagles medium (DMEM) – HG(High Glucose) and M-200 media specific for ECGM (Endothelial Cell Growth Media). Flow cytometry result of HUVECs of CD31 – 3% positivity, VE-Cadherin – 18.8% and vWF – 30.3%.References
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