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Design of Bioreactors for Tissue Engineering

Vikrant Singh, Rohit Yadav

Abstract


Utilizing ultra-scale down and microfluidic technology, micro and mini bioreactors are well characterised for usage in bioprocess research in from before the manufacture. Through use of bioreactors to study regular and pathophysiology, on the other hand, must be extremely distinct, and the physiological environment has an impact on bioreactor construction. The basic elements required for bioprocesses bioreactor to handle three major areas related to biological systems are examined in this review. All of these projects aim to recreate the in vitro model as accurately as possible so that they're being utilised to research cellular and molecular changes that occur physiology in order to develop tissue-engineered transplants for therapeutic use, at the molecular level, understanding disease pathogenesis, establishing potential therapeutic targets thus allowing adequate pharmaceutical testing on a truly realistic organoid, allowing for better medication design while also reducing the number of animals used in research. Also discussed is the use of bioreactor systems for the growth of clinically important types of cells. In contrast to cell growth, additional physical cues are required for the development of functioning three-dimensional tissue analogues. Bioreactors for musculoskeletal tissue engineering, as a result, are discussed.


Keywords


Bioreactors, Technology, Tissue Engineering, Therapeutics, Pathophysiology, cell signalling

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References


Martin I, Wendt D, Heberer M. The role of bioreactors in tissue engineering. Trends Biotechnol. 2004; 22: 80–86.

Carrier RL, Rupnick M, Langer R, et al. Perfusion improves tissue architecture of engineered cardiac muscle. Tissue Eng. 2002; 8: 175–188.

King JA, Miller WM. Bioreactor development for stem cell expansion and controlled differentiation. Curr Opin Chem Biol. 2007; 11: 394–398.

Crabbe A, Liu Y, Sarker SF, et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS ONE. 2015; 10: e0126846.

Kulig KM, Vacanti JP. Hepatic tissue engineering. Transpl Immunol. 2004; 12: 303–310.

Nahmias Y, Berthiaume F, Yarmush ML. Integration of technologies for hepatic tissue engineering. Adv Biochem Eng Biotechnol. 2007; 103: 309–329.

Wolff J. Das Gesetz der Transformation der Knochen. Hirshwald; Berlin, Germany: 1892.

Davis H. Conservative Surgery. Appleton. New York, NY, USA: 1867.

Gonzalez-Molina J, Selden BFC. Extracellular Fluid Viscosity Enhances Cell-Substrate Interaction and Impacts on Cell Size and Morphology. TCES, London, UK. 2016: 74.

McFetridge PS, Abe K, Horrocks M, et al. Vascular tissue engineering: Bioreactor design considerations for extended culture of primary human vascular smooth muscle cells. ASAIO J. 2007; 53: 623–630.

Groeber F, Engelhardt L, Lange J, et al. A first vascularized skin equivalent as an alternative to animal experimentation. Altex. 2016; 33: 415–422.

Egger D, Spitz S, Fischer M, et al. Application of a parallelizable perfusion bioreactor for physiologic 3D cell culture. Cells Tissues Organs. 2017; 203: 316–326

Weinbaum S, Cowin SC, Zeng Y. A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J Biomech. 1994; 27: 339–360.

Hung CT, Mauck RL, Wang CC, Lima EG, Ateshian G.A. A paradigm for functional tissue engineering of articular cartilage via applied physiologic deformational loading. Ann Biomed Eng. 2004; 32: 35–49.

Seidel JO, Pei M, Gray ML, et al. Long-term culture of tissue engineered cartilage in a perfused chamber with mechanical stimulation. Biorheology. 2004; 41: 445–458.

Liu C, Abedian R, Meister R, et al. Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds. Biomaterials. 2012; 33: 1052–1064.

Boccafoschi F, Botta M, Fusaro L, et al. Decellularized biological matrices: An interesting approach for cardiovascular tissue repair and regeneration. J Tissue Eng Regen Med. 2017; 11: 1648–1657.

Rana D, Zreiqat H, Benkirane-Jessel N, et al. Development of decellularized scaffolds for stem cell-driven tissue engineering. J Tissue Eng Regen Med. 2017; 11: 942–965.

Ma X, He Z, Li L, et al. Development and in vivo validation of tissue-engineered, small-diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells. J Cardiothoracic Surg. 2017; 12: 101.

Ghaedi M, Le AV, Hatachi G, et al. Bioengineered lungs generated from human ipscs-derived epithelial cells on native extracellular matrix. J Tissue Eng Regen Med. 2017.




DOI: https://doi.org/10.37628/ijibb.v8i1.762

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