Open Access Open Access  Restricted Access Subscription or Fee Access

Antimicrobial Activity of Waste Pulp of Citrus sinensis and Citrus limetta

Sukriti raj, Brahm Kumar Tiwari

Abstract


An antimicrobial is a substance which kills or inhibits the growth of different types of micro-organisms such as bacteria, fungi or protozoans. Citrus fruit products act as antimicrobial agents against the bacteria and the fungus. Citrus fruits belong to the family Rutaceae. The main objective was to determine the antimicrobial activity of Citrus sinensis waste pulp and to determine the antimicrobial activity of Citrus limetta waste pulp. The citrus pulp extracts showed a significant antibacterial activity against all the test organisms. The waste of C. sinensis pulp extracts showed a very good antibacterial activity when compared to the waste of C. limetta. Recycling of fruit waste is one of the most important ways of utilizing it in a number of novel products which are essentially required for human, animal and plant nutrition, as well as in the pharmaceutical industry. This work has evaluated the antibacterial activity in C. sinensis and C. limetta waste pulp extracts obtained from different solvents against different organisms.

Keywords: antimicrobial activity, Citrus limetta, Citrus sinensis
REFERENCES
[1] Amrita V, Sonal D, Shalini R. Antibacterial effect of herbs and spices extract on Escherichia coli. Electron J Biol. 2009; 5(2): 40–44p.
[2] Al-Ani WN, Al-Haliem SM, Tawfik NO. Evaluation of the antibacterial activity of citrus juices: an in vitro study. Al–Rafidain Dent J. 2009; 10(2): 376–382p.
[3] Bocco A, Cuvelier ME, Richard H, Berset C. Antioxidant activity and phenolic composition of citrus peel and seed extracts. J Agric Food Chem. 1998; 46: 212–213p.
[4] Baltch AL, Smith RP, Ritz WJ, Bopp LH. Comparison of inhibitory and bactericidal activities and postantibiotic effects of LY333328 and ampicillin used singly and in combination against vancomycin-resistant Enterococcus faecium. Antimicrob Agents Chemother. 1998; 42(10): 2564–2568p.
[5] Cotelle N, Bernier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM. Antioxidant properties of hydroxylflavones. Free Radic Biol Med. 1996; 20(1): 35–43p.
[6] Cheruvanky H. Method for treating hepercholesterolemia, hyperlipidemia and artherosclerosis. United States Path. 2004; 6(4): 733–799p.
[7] Ebrahimzadeh MA, Hosseinimehr SJ, Hamidinia A, Jafari M. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacol Online. 2008; 1: 7–14p.
[8] Elangovan V, Sekar N, Govindasamy S. Chemo-protective potential of dietary bioflavonoids against 20-methylcholanthreneinduced tumorigenesis. Cancer Lett. 1994; 87: 107–113p.
[9] Fernandez-Lopez J, Zhi N, Aleson-Carbonell L, Perez-Alvarez JA, Kuri V. Antioxidant and antibacterial activities of natural extracts: application in beef meatballs. Meat Sci. 2005; 69: 371–380p.
[10] Hammer KA, Carson C, Riley T. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol. 1999; 86(6): 985–990p.
[11] Harats D, Chevion S, Nahir M, Norman Y, Sagee O, Berry B. Citrus fruit supplementation reduces lipoprotein oxidation in young men ingesting a diet high in saturated fat: presumptive evidence for an interaction between vitamins C and E in vivo. Am J Clin Nutr. 1998; 67: 240–245p.
[12] Hayes AS, Markovic B. Toxicity of Beak housie citrodora. (Lemon Myrthle). Anti-microbial and in vitro cytotoxicity. Food Chem Toxicol. 2002; 40(4): 535–543p.
[13] Jayaprakasha GK, Girennavar B, Patil BS. Radical scavenging activities of Rio Red grapefruits and Sour orange fruit extracts in different in vitro model systems. Bioresour Technol. 2008; 99(10): 4484–4494p.
[14] Kumar KA, Subanthini A, Jayakumar M. Antimicrobial activity and phytochemical analysis of citrus fruit peels: utilization of fruit waste. Int J Engg Sci Technol. 2011; 3(6): 5414–5421p.
[15] Masuda G, Tomioka S. Quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method. Antimicrob Agents Chemother. 1978; 14(4): 587–595p.
[16] Okeke MI, Iroegbu CU, Eze EN, Okoli AS and Esimone CO. Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity. J Ethnopharmacol. 2001; 78(23): 119–127p.
[17] Okoli AS, Okeke MI, Iroegbu CU, Ebo PU. Antibacterial activity of Harungana madagascariensis leaf extracts. Phytother Res. 2002; 16(2): 174–179p.
[18] Rouseff RL, Perez-Cacho PR, Jabalpurwala F. Historical review of citrus flavor research during the past 100 years. J Agric Food Chem. 2009; 57: 8115–8124p.
[19] Rahman S, Parvez AK, Islam R, Khan MH. Antibacterial activity of natural spices on multiple drug resistant Escherichia coli isolated from drinking water, Bangladesh. Ann Clin Microb Antimicrob. 2011; 10: 10p.
[20] Stavric B. Antimutagens and anticarcinogens in foods. Food Chem Toxicol. 1993; 32: 79–90p.
[21] Sinclair WB, Ramsey RC. Changes in the organic acid content of Valencia oranges during development. Bot Gaz. 1994; 106: 140–148p.
[22] Sanders CC, Sanders WE Jr, Moland ES. Decimal assay for additivity of drugs permits delineation of synergy and antagonism. Antimicrob Agents Chemother. 1993; 37(2): 260–264p.
[23] Velu S, Abu Bakar F, Mahyudin NA, Saari N, Zaman MZ. In vitro antimicrobial activity of musk lime, key lime and lemon extracts against food related pathogenic and spoilage bacteria. Int Food Res J. 2014; 21(1): 379–386p.

Full Text:

PDF


DOI: https://doi.org/10.37628/jibb.v4i1.322

Refbacks

  • There are currently no refbacks.