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A CRISPR-Cas9 Mediated Gene editing in the Malaria Vector Mosquito

Mandeep Kaur

Abstract


Malaria effect millions of the people globally but with is introduction of Crispr cas 9 it could be curbed to greater extent. Crispr cas9 methods are transforming our ability to undertake functional genomics across a wide range of organisms, including malaria-causing Plasmodium parasites. Changing the genetics of entire animal populations may aid in the fight against illness. Gene disruption is a valuable tool for learning about the underlying biology of vector-pathogen interactions, and it may also be used to develop mosquito control techniques. However, embryonic insertion procedures for genetically manipulating mosquitos (particularly Anopheles) are challenging and ineffective, specifically in non-specialist facilities. Gene gets to drive to replace mosquito populations are promising technologies for malaria prevention. This review describes of the crispr cas9 gene editing, along the transposable elemental technology.

Keywords


Crispr cas9, Anopheles, plasmodium, effector genes, RNA’s, Transposable elements, Piggybac

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References


North R, Burt A, Charles H. Modelling the suppression of malaria vector using a CRISPR cas9

gene drive to reduce female fertility; BMC Biology. 2020; 18(98).

Manjit P, Ansuman P. Curbing Malaria: A New Hope through Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Technology. J Arthropod Borne Dis. 2017;11(4):539-540.

Zhang C, Xiao B, Jiang Y. Efficient editing of malaria parasite genome using CRISPR CAS9 system. mbio. 2014; 5(4): e01414-14.

Adolfi A, Gareth JL. Opening the toolkit for genetic analysis and control of Anopheles mosquito vectors. Current opinion in insect science. 2018; 30: 8-18.

Mang L, Akbari O, White B. Highly efficient site specific mutagensis in malaria mosquito using

crispr, Genes Genomics Genetics. 2017; 8(2):653-658.

Cargata P, Dong S, Dong Y, et al. Next geneeration tools to control Mosquito-transmitted disease. The annual review of microbiology. 2020; 74: 455-475.

Djogbénou VL, Jenkins AM, Regna K, et al. CYP6 P450 enzymes and ACE-1 duplication produce extreme and multiple insecticide resistance in the malaria mosquito Anopheles gambiae. Plos Genetics. 2014; 10(3): e1004236.

Lejarazú R, Jasinskiene N, James AA. Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi. Pnas. 2013; 110(18):7176-7181.

Barrangou R, Doudna JA. Applications of CRISPR technologies in research and beyond. Nat Biotechnol. 2016; 34: 933-941.

Gonzaga-Jauregui C, Lupski JR, Gibbs RA. Human genome sequencing in health and disease. Annu Rev Med. 2012; 63: 35-61.

Mali P, Yang L, Esvelt KM, et al. RNA-Guided Human Genome Engineering via Cas9. Science. 2013; 339(6121): 823-826.

Bhaya D, Davison M, Barrangou R. CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation. Annu Rev Genet. 2011; 45: 273-297.




DOI: https://doi.org/10.37628/ijger.v8i1.752

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