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Adaptation of Newcastle Disease Virus on Vero Cell Line

T. Rama Krishnan, Kirubanandan Shanmugam, Veera Subha

Abstract


ABSTRACT

A velogenic Newcastle disease virus isolate was passaged 10 times in Vero cell culture and the virus was assessed for the molecular changes associated with the passaging. At increasing passage levels, a gradual reduction in the virulence of the virus was observed. Molecular characterization of the virus was carried out by sequencing a portion of the fusion gene (374 bp) encompassing the fusion protein cleavage site (FPCS), which was previously amplified by reverse transcription–polymerase chain reaction. Sequence analysis revealed a total of 31 nucleotide substitutions which resulted in the change of 16 amino acids between the velogenic virus and the 10th passage virus. The predicted amino acid motif present at the cleavage site of the virulent virus was 109SRRRRQRRFVG119 and the corresponding region of the adapted virus was 109SGGRRQKRFIG119. Thus, the assessing of molecular changes before and after adaptation of velogenic Newcastle disease virus to Vero cells was studied.

 

Keywords: Newcastle disease virus, Vero cells, adaptation, fusion gene, sequencing studies

Cite this Article: T. Rama Krishnan, Kirubanandan Shanmugam, Veera Subha. Adaptation of Newcastle Disease Virus on Vero Cell Line. International Journal of Cell Biology and Cellular Processes. 2020; 6(2): 43–74p.


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