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Next generation bioluminescence imaging technology for tracking numerous biological processes under in vivo conditions

Sakshi Tewari, Shalini Sharma

Abstract


To expand our understanding on numerous biological processes happening inside living entities, the mechanism of bioluminescence exists. The process of visible light emission in living things is referred to as bioluminescence. Small laboratory animals can now be molecularly imaged using bioluminescence imaging. It is a potent technology that has been developed over the past ten years in diverse fields of science. In the area of virology, immunology, oncology, and neuroscience, this technology offers efficient ways for tracking numerous in vivo biological processes. The technique utilizes the enzyme luciferase, which is transferred inside of tissues or an entire organism, allowing for the tracking of cells, observation of gene expression, and protein-protein interactions. Despite the fact that fluorescence reporters and sensors are being used more frequently in a variety of industries, their application has been constrained by thick tissues and living entities. This problem has been solved by BLI via developing bioluminescence reporters and sensors that use enzyme-catalyzed processes to produce light emitters. The article begins with the description of diverse luciferase and luciferin pairs, followed by bioluminescent systems, their methodology, applications and ongoing trends. The paper provides details on the bioluminescence imaging methodology and its numerous applications. It emphasizes on how this method can be blended with other techniques and can serve as next generation technology for tracking numerous biological processes under in vivo conditions.


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Zinn, K.R., Chaudhuri, T.R., Szafran, A.A., O'Quinn, D., Weaver, C., Dugger, K., Lamar, D., Kesterson, R.A., Wang, X. and Frank, S.J. Noninvasive bioluminescence imaging in small animals. ILAR Journal; 2008. 49 (1): 103–115.

Sureda-Vives, M. and Sarkisyan, KS. Bioluminescence-driven optogenetics. Life; 2020. 10 (12): 318.

Badr, C.E. Bioluminescence imaging: basics and practical limitations. Bioluminescent Imaging; 2014. 1–18.

Sharifian, S., Homaei, A., Hemmati, R., Luwor, R.B., & Khajeh, K. The emerging use of bioluminescence in medical research. Biomedicine & Pharmacotherapy; 2018. 101: 74–86.

Hall, M.P., Unch, J., Binkowski, B.F., Valley, M.P., Butler, B.L., Wood, M.G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T. and Robers, M.B. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chemical biology; 2012. 7 (11): 1848–1857.

Syed, A.J., & Anderson, J.C. Applications of bioluminescence in biotechnology and beyond. Chemical Society Reviews; 2021. 50 (9): 5668–5705.

Tannous, B.A., Kim, D.E., Fernandez, J.L., Weissleder, R. and Breakefield, X.O. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Molecular Therapy; 2005. 11 (3): 435–443.

Bhaumik, S., & Gambhir, S.S. Optical imaging of Renilla luciferase reporter gene expression in living mice. Proceedings of the National Academy of Sciences; 2002. 99 (1): 377–382.

Inouye, S., Noguchi, M., Sakaki, Y., Takagi, Y., Miyata, T., Iwanaga, S., Miyata, T. and Tsuji, F.I. Cloning and sequence analysis of cDNA for the luminescent protein Aequorin. Proceedings of the National Academy of Sciences; 1985. 82 (10): 3154–3158.

Tanahashi, H., Ito, T., Inouye, S., Tsuji, F.I. and Sakaki, Y. Photoprotein aequorin: use as a reporter enzyme in studying gene expression in mammalian cells. Gene; 1990. 96 (2): 249–255.

Miloud, T., Henrich, C. and Hammerling, G.J. Quantitative comparison of click beetle and firefly luciferases for in vivo bioluminescence imaging. Journal of Biomedical Optics; 2007. 12 (5): 054018.

Zhao, H., Doyle, T.C., Coquoz, O., Kalish, F., Rice, B.W. and Contag, C.H. Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo. Journal of Biomedical Optics; 2005. 10 (4): 41210.

Branchini, B.R., Ablamsky, D.M., Murtiashaw, M. H., Uzasci, L., Fraga, H., & Southworth, T.L. Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications. Analytical Biochemistry; 2007. 361 (2): 253–262.

Vieira, J., da Silva, L.P. and da Silva, J.C.E. Advances in the knowledge of light emission by firefly luciferin and oxyluciferin. Journal of Photochemistry and Photobiology B: Biology; 2012. 117:

–39.

Baldwin, T.O., Berends, T., Bunch, T.A., Holzman, T.F., Rausch, S.K., Shamansky, L., Ziegler, M.M. Cloning of the luciferase structural genes from Vibrio harveyi and expression of bioluminescence in Escherichia coli. Biochemistry; 1984. 23 (16): 3663–3667.

Nakajima, Y., Kobayashi, K., Yamagishi, K., Enomoto, T. and Ohmiya, Y. cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca. Bioscience, Biotechnology, and Biochemistry; 2004. 68 (3): 565–570.

Stacer, A.C., Nyati, S., Moudgil, P., Iyengar, R., Luker, K.E., Rehemtulla, A. and Luker, G.D. NanoLuc reporter for dual luciferase imaging in living animals. Molecular Imaging; 2013. 12 (7): 7290–2013.

Navizet, I., Liu, Y. J., Ferré, N., Roca‐Sanjuán, D., & Lindh, R. The chemistry of bioluminescence: an analysis of chemical functionalities. ChemPhysChem; 2011. 12 (17): 3064–3076.

Wilson, T. Bioluminescence: living lights, lights for living. Harvard University Press; 2013.

Mezzanotte, L., van‘t Root, M., Karatas, H., Goun, E.A. and Löwik, C.W. In vivo molecular bioluminescence imaging: new tools and applications. Trends in Biotechnology; 2017. 35 (7):

–652.

Contag, C.H., & Bachmann, M.H. Advances in in vivo bioluminescence imaging of gene expression. Annual review of biomedical engineering; 2002. 4 (1): 235–260.

Sadikot, R.T., & Blackwell, T.S. Bioluminescence imaging. Proceedings of the American Thoracic Society; 2005. 2 (6): 537–540.

Ray, P. and Gambhir, S.S. Noninvasive imaging of molecular events with bioluminescent reporter genes in living subjects. Reporter Genes; 2007. 131–144.

Bhang, H.E. et al. Tumor-specific imaging through progression elevated gene-3 promoter-driven gene expression. Nat. Med; 2011. 17 (1): 123–129.

Parag-Sharma, K., O’Banion, C.P., Henry, E.C., Musicant, A.M., Cleveland, J.L., Lawrence, D.S., & Amelio, A.L. Engineered BRET-based biologic light sources enable spatiotemporal control over diverse optogenetic systems. ACS synthetic biology; 2020. 9 (1): 1–9.

Niers, J.M. et al. Multimodal in vivo imaging and blood monitoring of intrinsic and extrinsic apoptosis. Mol. Ther; 2011. 19 (6): 1090–1096

Contag, C.H., Contag, P.R., Mullins, J.I., Spilman, S.D., Stevenson, D.K., & Benaron, D.A. Photonic detection of bacterial pathogens in living hosts. Molecular Microbiology; 1995. 18 (4): 593–603.

Prescher, J.A. and Contag, C.H. Guided by the light: visualizing biomolecular processes in living animals with bioluminescence. Current opinion in Chemical Biology; 2010. 14 (1): 80–89.

Reynolds, J.S., Troy, T.L., Mayer, R.H., Thompson, A.B., Waters, D.J., Cornell, K.K., Snyder, P.W. and Sevick‐Muraca, E.M. Imaging of spontaneous canine mammary tumors using fluorescent contrast agents. Photochemistry and Photobiology; 1999. 70 (1): 87–94.

Rice, B.W., Cable, M.D. and Nelson, M.B. In vivo imaging of light-emitting probes. Journal of Biomedical Optics; 2001. 6 (4): 432–440.




DOI: https://doi.org/10.37628/ijaba.v8i2.831

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