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Evaluation of Cytotoxic Activity of Cestrum nocturnum Leaves Extract against A549 Cell Line using MTT Assay

Prachi Sharma

Abstract


Cancer remains a major global cause of death, necessitating ongoing research for effective treatments. Traditional herbal medicine is being explored for novel anti-cancer compounds. This study investigates the cytotoxic effects of Cestrum nocturnum ethanolic extract on A549 lung cancer cells, a type of non-small cell lung cancer (NSCLC). NSCLC is a significant health concern in India, affecting even non-smokers. Cestrum nocturnum, known as Night-blooming jasmine, has shown promise in inhibiting tumor growth in mice, and its active compound, apigenin, exhibits anti-cancer properties. Apigenin modulates epigenetic mechanisms involved in cancer development and progression, including DNA methylation, histone modification, signaling pathways, cell cycle arrest, and apoptosis. The ethanolic extract demonstrated concentration-dependent cytotoxicity, indicating potential anti-cancer properties. This study underscores Cestrum nocturnum's potential as an anti-cancer agent and provides insights into apigenin's mechanisms of action, warranting further research for therapeutic applications in NSCLC treatment.


Keywords


A549, MTT assay, Cestrum nocturnum, lung cancer

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References


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