In-vitro plant regeneration of Mulberry (Morus alba L.)

Abstract

The present investigation entitled “In-vitro plant regeneration of Mulberry (Morus alba L.)” was conducted at Plant Tissue Culture Laboratory of K. K. Wagh College of Agricultural Biotechnology, Nashik, Maharashtra, India. Development of improved protocols for the regeneration of Morus alba L. is important for their commercial utilization. The present study was initiated to optimize in-vitro propagation protocol from nodal culture of Mulberry. Three surface sterilization treatment were used, the nodal segment was treated with 0.8% Bavistin for 15 mins, followed by 0.1% mercuric chloride for 5 min and finally treated with 70% ethanol for 30 sec. throughout the experiment with minimum contamination. The nodes were cultured on MS medium supplemented with different concentrations of BAP (0-1.5 mg/l) and same concentration of NAA (0.2 mg/l) were used. The maximum percentage of node-initiated shoots (90%) were observed on MS media supplemented with BAP (1.25 mg/l) combined with NAA (0.2 mg/l) after 3 weeks, whereas same concentration was best for multiplied shoots (90%) after 6 weeks. The rooting was attempted on MS media supplemented with IBA (0.2 mg/l).