Studies on Extraction, Modelling and Purification of Quercetin from Peel of Allium cepa

Abstract

ABSTRACT
Quercetin is an anti-oxidant, which very efficiently scavenges highly reactive biological species such as peroxynitrite and hydroxyl radical. Quercetin belongs to the flavonoid’s family. The present studies on optimization of physico-chemical parameters like effects of different solvents, different solvent percentages, particle size, pH and extraction time for the extraction of quercetin was studied. The highest quercetin concentration for optimized conditions was 38 µg/ml. In the soxhlet extraction process, the extraction of quercetin concentration was 52 µg/ml obtained at 4 hours. This extract was drawn in to the Column chromatography and its concentration was increased to 57.0 µg/ml. The purity of quercetin was improved by column chromatography at 30 min. In the liquid–liquid extraction process, partition coefficient of quercetin was found to be 3.57 and it was carried out using ethanol (raffinate phase) and hexane (extract phase) solvents at different extraction times to verify the mathematical model proposed in this work. The final form of the proposed models were E(t) = –0.000t2 + 0.145t + 14 for quercetin where Es= yield extract (µg/ml of quercetin) and t = extraction time (min).

Keywords: Allium cepa, extraction, partition coefficient, purification, quercetin
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