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Molecular Markers: Roles and its Applications

Sujata Poornima, Heena Khan, Ashwin Kashyap

Abstract


The rapid advancement of genomic technologies has prepared the way for genomes. It has created new molecular marker variants for use in genetic manipulation of farm animals. Several of the primary molecular or DNA markers typically employed in plant research have been described, along with their principles, techniques, and applications. The fundamental principles of molecular marker techniques have also been clarified, as well as a thorough explanation of some key fundamental concepts connected to marker use. By using marker-assisted selecting, DNA markers improved the productivity and precision of traditional Marker plant breeding (MAS). Plenty of the molecular approaches for detecting variation that have been developed over the years do so under one of three conceptually distinct types of markers: protein variations, DNA variants, and RNA variants (allozymes). It really has developed novel molecular marker generations for application in farm animal genetic improvement. Such markers provide further precise genomic data and a good knowledge of the genetic resources of animals.


Keywords


DNA marker, Molecular marker, Genome, Polymorphism, Genetic transformation

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References


Shanti ML, Shenoy VV, Devi GL, et al. Marker-assisted breeding for resistance to bacterial leaf blight in popular cultivar and parental lines of hybrid rice. J Plant Pathol. 2010; 92 (2): 495–501.

Elshire RJ, Glaubitz JC, Sun Q, et al. A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS One. 2011; 6 (5): e19379.

Peterson BK, Weber JN, Kay EH, et al. Double digest RADseq: an inexpensive method for de novo SNP discovery and genotyping in model and non-model species. PLoS One. 2012; 7 (5): e37135.

Bourgeois Y, Boissinot S. On the population dynamics of junk: A review on the Population Genomics of Transposable Elements. Genes. 2019; 10 (6): 419.

Gao B, Shen D, Xue S, et al. The contribution of transposable elements to size variations between four teleost genomes. Mob DNA. 2016; 7 (1): 4.

Morgante M, Brunner S, Pea G, et al. Gene duplication and exon shuffling by helitron-like transposons generate intraspecies diversity in maize. Nat Genet. 2005; 37 (9): 997–1002.

Negi P, Rai AN, Suprasanna P. Moving through the stressed genome: emerging regulatory roles for transposons in plant stress response. Front Plant Sci. 2016; 7: 1448.

Roy NS, Choi JY, Lee SI, et al. Marker utility of transposable elements for plant genetics, breeding, and ecology: a review. Genes Genom. 2015; 37 (2): 141–151.

Fan F, Cui B, Zhang T, et al. LTR-retrotransposon activation, IRAP marker development and its potential in genetic diversity assessment of masson pine (Pinus massoniana) Tree Genet Genomes. 2014; 10(1): 213–222.

Agarwal M, Shrivastava N, Padh H. Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Rep. 2008; 27 (4): 617–631.

Jing R, Vershinin A, Grzebyta J, et al. The genetic diversity and evolution of field pea (Pisum) studied by high throughput retrotransposon-based insertion polymorphism (RBIP) marker analysis. BMC Evol Biol. 2010; 10 (1): 44.

Wenke T, Seibt KM, Döbel T, et al. Plant Genotyping. New York: Humana Press; 2015. Inter-SINE amplified polymorphism (ISAP) for rapid and robust plant genotyping; pp. 183–192.

Kalendar R, Antonius K, Smýkal P, et al. iPBS: a universal method for DNA fingerprinting and retrotransposon isolation. Theor Appl Genet. 2010; 121 (8):1419–1430.

Monden Y, Fujii N, Yamaguchi K, et al. Efficient screening of long terminal repeat retrotransposons that show high insertion polymorphism via high-throughput sequencing of the primer binding site. Genome. 2014; 57 (5): 245–252.

Ramirez-Prado JS, Abulfaraj AA, Rayapuram N, et al. Plant immunity: from signaling to epigenetic control of defense. Trends Plant Sci. 2018; 23 (9): 833–844.

Sarris PF, Cevik V, Dagdas G, et al. Comparative analysis of plant immune receptor architectures uncovers host proteins likely targeted by pathogens. BMC Biol. 2016;14(1):8. doi: 10.1186/s12915-016-0228-7.

Saijo Y, Loo EPI, Yasuda S. Pattern recognition receptors and signaling in plant–microbe interactions. Plant J. 2018; 93 (4): 592–613.

Gebhardt C, Valkonen JP. Organization of genes controlling disease resistance in the potato genome. Annu Rev Phytopathol. 2001; 39 (1): 79–102.

Adjebeng DJ, Manu-Aduening J, Asante IK, et al. Genetic diversity and population structure analysis of Ghanaian and exotic cassava accessions using simple sequence repeat (SSR) markers. Heliyon. 2020; 6 (1): e0315.

Akash MW, Shiyab SM, Saleh MI. Yield and AFLP analyses of inter-landrace variability in okra (Abelmoschus esculentus L.). Life Sci J. 2013; 10 (2): 2771–2779.

Alghamdi S, Al-Faifi S, Migdadi H, et al. Molecular diversity assessment using sequence related amplified polymorphism (SRAP) markers in Vicia faba L. International Journal of Molecular Sciences. 2012; 13: 16457–16471.

Ali EM, Tohidfar M, Karimi M, et al. Determination of genetic uniformity in transgenic cotton plants using DNA markers (RAPD and ISSR) and SDS-PAGE. Journal of Plant Molecular Breed. 2015; 3 (2): 36–43.


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