Agarose gel electrophoresis   a tool for DNA separation

Teena Agrawal

Agarose gel electrophoresis a tool for DNA separation

Teena Agrawal

Abstract

Agarose gel electrophoresis is used for the separation of the DNA fragment based on the molecular sizes. Small fragments migrate than the larger ones. It is the most effective way for the separation of the DNA based on the sizes ranging from the 110 bp to 26 kb1. Agarose’s is isolated from the sea weeds Gelidium, and Gracilaria. The agarose is made-up of the repeating unit of the agar, in this practical gel is casted on plates and well are formed, in well DNA and dyes are added, by the electrophoresis DNA is separated based on the sizes, by the electrophoresis by the electronic voltages. the rate of movement of the DNA depends on the several factors, some of the factors are followed as (molecular Sizes of DNA molecules 2) Agarose concentration 3) DNA orientation 4) Combination of the factors in appropriate way. After separation DNA molecules are visualized by the stain on the gel doc apparatus.

Keywords

Agarose gel electrophoresis, Agarose’s Gelidium, and Gracilaria, DNA molecules, Gel doc apparatus.

Full Text:

PDF

References

Sambrook, J., 2001. Russell DW Sambrook J, Russell DW. Molecular cloning.

Kirkpatrick, F.H., 1990. Overview of agarose gel properties. Current communications in cell & molecular biology.

Helling, R.B., Goodman, H.M. and Boyer, H.W., 1974. Analysis of endonuclease R· Eco RI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. Journal of virology, 14 (5), pp. 1235–1244.

Smith, S.B., Aldridge, P.K. and Callis, J.B., 1989. Observation of individual DNA molecules undergoing gel electrophoresis. Science, 243 (4888), pp. 203–206.

https://universe84a.com/collection/gel-electrophoresis/

Aaij, C. and Borst, P., 1972. The gel electrophoresis of DNA. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis, 269 (2), pp. 192–200.

Lai, E., Birren, B.W., Clark, S.M., Simon, M.I. and Hood, L., 1989. Pulsed field gel electrophoresis. Biotechniques, 7 (1), pp. 34–42.

Lee, P.Y., Costumbrado, J., Hsu, C.Y. and Kim, Y.H., 2012. Agarose gel electrophoresis for the separation of DNA fragments. JoVE (Journal of Visualized Experiments), (62), p.e3923.

Serwer, P., 1983. Agarose gels: properties and use for

electrophoresis. Electrophoresis, 4 (6), pp. 375–382.

Dea, I.C.M., McKinnon, A.A. and Rees, D.A., 1972. Tertiary and quaternary structure in aqueous polysaccharide systems which model cell wall cohesion: reversible changes in conformation and association of agarose, carrageenan and galactomannans. Journal of Molecular Biology, 68 (1), pp. 153–172.

Sharp, P.A., Sugden, B. and Sambrook, J., 1973. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry, 12 (16), pp. 3055–3063.

Chen, H., Jarosch, K.A., Mészáros, É., Frossard, E., Zhao, X. and Oberson, A., 2021. Repeated drying and rewetting differently affect abiotic and biotic soil phosphorus (P) dynamics in a sandy soil: A 33P soil incubation study. Soil Biology and Biochemistry, 153, p.108079.

Giot, J.F., 2010. Agarose gel electrophoresis: Applications in clinical chemistry. Journal of Medical Biochemistry, 29 (1), pp. 9–14.

Mansour, F.H. and Pestov, D.G., 2013. Separation of long RNA by agarose–formaldehyde gel electrophoresis. Analytical biochemistry, 441 (1), pp. 18–20.

Refbacks

There are currently no refbacks.