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Optimization of Physicochemical Parameters for Extraction of Total Phenolic Content from Composition of Peel and Seed of Cucurbita Species

Disha Kumari, Davneet Kaur, Aman Srivastava, Surya Prakash D.V.

Abstract


In this work, various parameters are used for the drawing out of phenolic compounds from peel and seed of cucurbita and estimation of Total Phenolic Content (TPC) by using FC reagent method. The optimization of physico-chemical parameters like effects of different solvents, different solvent percentages, particle size, pH and extraction time for the extraction of TPC was studied. For the extraction of TPC, the optimum results were observed for the effects of different solvents, different solvent percentages, particle size, pH and extraction time for the extraction of TPC were ethylacetate, 65%, 125 microns, 5.0 and 24 hr respectively. The highest TPC concentration for optimized conditions was 15µg/ml.
In this study, various parameters are used for the drawing out of phenolic compounds from peel and seed of Cucurbita and estimation of total phenolic content (TPC) by using FC reagent method. The optimization of physicochemical parameters like effects of different solvents, different solvent percentages, particle size, pH and extraction time on the extraction of TPC was studied. For the extraction of TPC, the optimum results were observed for the effects of different solvents such as ethyl acetate: the solvent percentages, particle size, pH and the extraction time on the extraction of TPC were 65%, 125 microns, 5.0 and 24 h, respectively. The highest TPC concentration for optimized conditions was 15 µg/ml.

Keywords: Cucurbita, ethyl acetate, FC reagent, optimization, total phenolic content.

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References


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DOI: https://doi.org/10.37628/ijbb.v4i2.355

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