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Studies on Extraction and Purification of Total Phenolic Content from Seeds of Carica papaya

Anurag Agarwal, Ankit Rawat Rawat, Priyanka Bhat, surya prakash dv

Abstract


Carica papaya is belongs to Caricaceae family and it contains high content of phenolic compounds and medicinal properties. It shows various pharmacological activities like anti-oxidant, anti-cancer, anti-diabetic, anti-inflammatory, wound healing activities. Here in this research work, for the extraction of TPC from seeds of Carica papaya, the optimum results were observed for the effects of different solvents, solvent percentages, pH, extraction time and particle size for the extraction of TPC were ethanol, 100%, 6.0, 24hr and 120 mesh size respectively. The highest TPC concentration for optimized conditions was 27µg/ml. In the Soxhlet extraction process, the extraction of TPC concentration was 42µg/ml obtained from seeds of papaya at 4hr. This extract was drawn in to the Column chromatography and its concentration was increased to 45µg/ml at 30min.


Keywords


Extraction, Purification, Total Phenolic Content, Carica papaya, Ethanol

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