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Analysis of Viability Index and Growth of Regenerated Cyanobacteria Following Long-Term Preservation in Calcium Alginate Beads

Tridip Phukan, Rabbul Ibne A. Ahad, Mayashree B. Syiem

Abstract


Samples were collected from Nagaon and Dhemaji districts of Assam differing significantly in pH and temperature for cyanobacterial isolation. Isolates were partially characterized, and pure cultures of the isolates were immobilized for preservation. Thirty two cyanobacterial strains were isolated from 13 different soil and water samples. Nostoc was the most abundant genus in the collection (42%). This was followed by Anabaena (22%), Fischerella (19%), Synechococcus (6%), and Calothrix (5%). For partial characterization increase in chlorophyll a and in protein content was taken as measurement of growth. Diazotrophic growth was assessed by analyzing heterocyst frequency and nitrogenase activities. Maximum nitrogenase activity was seen in Anabaena isolates. Anabaena sp.1, 9, 6, 7 and Nostoc sp.7 showed nitrogenase activity within the range of 1.97–1.85 nmol C2H2 reduced/μg chl a/h. These isolates projected good biofertilizer potential. All cyanobacterial isolates were preserved in the calcium alginate beads for long-term preservation. Quarterly 10 preserved beads were resuspended in the liquid media and their viability and growth were measured and compared to their control cultures to establish the suitability of the preservation technique in preserving the cyanobacterial germplasm. All isolates that were preserved maintained more than 68% viability under storage for 1 year in the calcium alginate beads making this protocol an effective way of long-term preservation of cyanobacteria.

Keywords


abundance, cyanobacteria, growth, heterocyst frequency, immobilization

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DOI: https://doi.org/10.37628/jibb.v1i2.40

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