Bioremediation of Oil-Spilled Sites through Seeding Naturally Adapted Pseudomonas putida

Abstract

In the present study, crude oil degradation was analyzed using isolated bacterial strains. These bacterial strains were isolated from the crude-oil-contaminated soil sample. All the isolates were identified as species of Pseudomonas, in which the Pseudomonas putida P11 and LP1 demonstrated a strong ability to degrade kerosene, gasoline, diesel, engine oil and crude oil. Bioremediation for oil spill cleanup was analyzed by the inoculation of P. putida with the addition of appropriate inorganic nutrients, which may be a suitable method. This method is used for a rapid rehabilitation of agricultural land upon pollution with crude petroleum. Their ability to degrade hydrocarbon contamination in the environment was investigated using soil samples that were contaminated with diesel, crude oil or engine oil. The Pseudomonas strain LP1, an organism isolated on the basis of its ability to grow on pyrene, was assayed for its degradative potentials when growing on crude, diesel and engine oils. Replicate trials comprising oil, oil plus bacteria, oil plus fertilizer, and oil plus bacteria and fertilizer were monitored for total viable count and rate of degradation of crude oil.

Keywords: biodegradation, bio-surfactants, engine oil, polycyclic aromatic hydrocarbons, Pseudomonas
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