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Isoenzyme Profiling of Polyphenoloxidase and Phenylalanine Ammonia Lyase in Cytoplasm of Lycopersicum esculentum in Response to Leaf Extract of Tagetes erecta (Marigold)

Ankita singh

Abstract


In the present study, the effect of leaf extracts of marigold (Tagetes erecta ) on the activity of Polyphenoloxidase (PPO) in Tomato (Lycopersicum esculentum) leading to the changes in the expression of the PR proteins Polyphenol oxidase (PPO) and Phenyl Ammonia Lyase (PAL) was evaluated. For this evaluation four sampling intervals were taken 0 hour, 24 hour,
48 hour and 72 hour. At the third node from base of each plant leaves were treated and samples from the distal untreated leaves were collected. The change in the activity of defense enzyme PPO and PAL and the expression of PPO isoforms was studied. The results demonstrate Seven acidic isoforms of PPO (Rf = 0.37, 0.5, 0.61, 0.65, 0.69, 0.71 and 0.74) could be observed in both control and marigold extract treated plants.

Keywords


Lycopersicum esculentum, phenyl ammonia lyase, polyphenol oxidase, PR proteins, Tagetes erecta

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References


Almagro L, Go´mezRos LV, Belchi- Navarro S, Bru R, RosBarcelo´ A and Pedren˜ MA (2009). Class III peroxidases in plant defence reactions.

Journal of Experimental Botany 2009; Vol. 60, No. 2, pp. 377–390.

Stangarlin JA, Kuhn OJ, Assi L and Schwan-Estrada KRF. Control of plant diseases using extracts from medicinal plants and fungi. Science against microbial pathogens: communicating current research and technological advances A. Méndez-Vilas (Ed.) 2011; 1033-1042.

Azad MAK, Yesmin MN and Islam MS. 2012. Effect of Botanical Extract

on Pest Control in Brinjal Field. J. Environ. Sci. & Natural Resources,

(2): 173 – 176.

Chakrabarty PK, Mukewar PM, Raj S, Sravan Kumar,V. Biochemical factors governing resistance in diploid cotton against grey mildew. Indian

Phytopathology 2002; 55(2):140-146.

Boussaada O, Kamel MBH, Ammar, HAOUAS D, Mighri Z, Helal AN.2008.

Ali A, Rizvi PQ, Khan FR. 2010. Bioefficacy of some plant leaf extracts

against mustard aphid, Lipaphiserysimikalt on indian mustard, Brassica juncea. Journal of plant protection research. Vol. 50, No. 2. 130-132.

Sigh UP and Prithviraj B. Neemazal, a product of neem (Azadirachtaindica), induces resistance in pea (Pisumsativum) against Erysiphepisi. Physiological and Molecular Plant Pathology 1997; Volume 51, Issue 3, Pages 181–194.

Chelikani P, Fita I, Loewen PC (2004). Diversity of structures and properties among catalases. Cell and Molecular Life Sciences 61, 192–208.

Vimala R, Suriachandraselvan M. Induced resistance in bhendi against

powdery mildew by foliar application of salicylic acid. Journal of

Biopesticides 2009; 2(1):111-114.

Velazhahan R. Induction of phenolics and defense-related enzymes in coconut (Cocosnucifera L.) roots treated with biocontrol agents,

Brazilian Journal of Plant Physiology 2006; 18(3):367-377.

Kavitha R and Umesha S. Regulation of Defense-Related Enzymes

Associated with Bacterial Spot Resistance in Tomato. Phytoparasitica 2008; 36(2): 144-159.

Németh ZI, Pozsgai-Harsányi M, Gálos B, Levente A. Stress Sensitivity of Correlation between POD and PPO Activities in Plants. ActaSilv. Lign. Hung. 2009; Vol. 5: 27-45.

Wu HY, Duan YX. Defense response of soybean (Glycine max) to soybean cyst nematode (Heteroderaglycines) race 3 infection. 2011; v. 21(2): p. 165- 170.

Richter C, Dirks ME, Gronover CS, Prüfer D, Moerschbacher BM.

Silencing and heterologous expression of PPO-2 indicate a specific function of a single polyphenol oxidase isoform in resistance of dandelion

(Taraxacumofficinale) against Pseudomonas syringaepv. tomato. Mol

Plant Microbe Interact. 2012 Feb;

Marimuthu T, Kagale J, Thayumanavan B, Nandakumar R and

Samiyappan R. Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Daturametel against Rhizoctoniasolani and Xanthomonasoryzae pv. oryzae. Physiological and Molecular Plant Pathology 2004; Volume 65, Issue 2, Pages 91– 100.




DOI: https://doi.org/10.37628/ijger.v2i1.72

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