Sequencing based strategies for the diagnosis of oral cancer

Abstract

Oral cancer is a multifactorial disease and is widely prevalent among cancer types. It is emerging as a rapidly growing problem due to tobacco, alcohol, inappropriate nutritional diet, exposure to toxic chemicals, and HPV infections. Oral squamous cell carcinoma (OSCC) is the most frequent of all oral neoplasms. It is the leading cause of death and the most common cancers and it constitutes a major health problem particularly in developing countries. Early detection can minimize its detrimental effects and can improve the quality of life of the patient by examination and treatment. The aim of this review article is to identify the advanced sequencing technology compared to conventional methods in oral cancer diagnosis. Sequencing methods are a high chance of positive if the sample is pure and without contaminants. In addition to these sequencing methods are advanced, rapid, sensitive, specific, accurate compared to screening checks that are examined and treated earlier. Next-generation sequences (NGS) are highly cost-effective and intensive work required; traditional advanced sequencing was only performed on specific DNA regions and for a specific suspected sample. Due to accuracy, people will think that cost-effectiveness can reduce the cost per sample to make it a more attractive platform in the market for these kinds of sequencing modalities.

Keywords: Diagnosis, HPV16, NGS, Oral Cancer, Sequencing methods

Cite this Article: Vinay Kumar Thotla, Gopal Purohit, Subhashree Mishra, Swadhin Palo, Pratyush Kumar Das. Sequencing based strategies for the diagnosis of oral cancer. International Journal of Genetic Engineering and Recombination. 2020; 6(2): 24–32p.