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Comparative Study of In-Vitro Antibacterial Activity and Determination of Phytochemical Constituents of Some Selected Plants (Catharanthus roseus, Eclipta alba and Azadirachta indica)

Daisy Nanda, Seema Dwivedi

Abstract


Medicinal plants are valuable natural resources that are effective against various pathogenic bacteria and infectious bacteria and they are considered as a rich source of nutrition as well as a raw material for extraction of active ingredients that are present in it. The potential for developing antimicrobial from higher plants appears to be important as it will lead to development of a phytomedicine to act against pathogenic microbes. Therefore, medicinal plants are one of the versatile source for modern medicine to attain new principles. Medicinal plant based antibacterial have large number of therapeutic potential as they help in a natural way without causing any side effects like synthetic drugs. Thus, based on taxonomy and pharmacological information, antibacterial activities of three medicinal plants that are C. roseus, E. alba and A. indica were evaluated in different extracts (ethanol, methanol and aqueous) against four microorganisms that were Escherichia coli, Psuedomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The method used for determination of antibacterial activity were performed by agar well diffusion method. Results showed that ethanol extract of Eclipta alba exhibited strong antibacterial activity against all four bacteria and at all concentrations (25%, 50%, 75% and 100%). Antibacterial activity of methanol extract of Eclipta alba, Catharanthus roseus and Azadirachta indica found to be moderate against bacteria at all concentrations whereas aqueous extract of exhibited least antibacterial activity.

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