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Type 1 Collagen Gels as a Three-dimensional Cell Culture System for Dendritic Cell

Aishwarya Satish, Kaarunya Sampthkumar, Veera Subha, Kirubanandan Shanmugam


Type 1 Collagen was extracted from Bovine Tendons and Rat Tails for developing gels for the cultivation of Dendritic cells. The Type 1 Collagen gels play as a scaffold for cellular microenvironment for the growth of Dendritic cells. The effect of agarose in the collagen gels on the DC growth were investigated. The dendritic cell populations were isolated from monocytes in peripheral blood and were used to seed the collagen-agarose scaffold and the growth of DC were consistent with previously reported work. Dendritic cell maturation was studied in the presence and absence of collagen. In the absence of collagen, the individual cells developed visible dendrites after 6–7 days of culture and clusters of dead cells were seen after 10 days of culture. The Type 1 Collagen promotes further mature of immature DC. The Triple Helix Conformation of Type 1 Collagen and three-dimensional nature of gels as scaffold contributes the growth and maturation of DC into the tissue. Given this correspondence, Type 1 Collagen matures DC and it can be good alternative for synthetic polymer-based 3D cell culture systems.


Type 1 Collagen, Agarose, Scaffold, Dendritic Cells, Maturation and Bovine Tendons

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