Anticancer Activity of Methanol Extract of Crataeva nurvala in HeLa cell Line

Abstract

Synthesis and characterization of anticancer activity of methanol extract of Crataeva nurvala in HeLa cell line free from infection was collected from Kallakurichi District in Tamil Nadu. Roots were used to prepare extracts. The roots collected were ached with water to remove the soil and dust particles. The nanoparticle was characterized by (FT-IR) spectroscopy analyse is used to analyse the functional groups present in a molecule. The GC-MS analysis was carried out on a GC Clarus 500 Perkin Elmer system and Gas Chromatograph interfaced to a Mass Spectrometer (GC-MS) instrument employing the following conditions from spectrum of the unknown component was compared with the spectrum of the known components and name, molecular weight and structure of the components of the tested materials were ascertained. The hydroxyl radical scavenging activity of C. nurvala was determined by the method of (Halliwell et al., 1987). In this assay, OH• has been generated by reduction of H2O2 the transition metal (iron) in the presence of ascorbic acid, Superoxide anion radical scavenging activity, Effect of Crataeva nurvala on DPPH scavenging activity, Effect of Crataeva nurvala on ABTS scavenging activity, Effect of Crataeva nurvala on apoptotic morphological changes was reported.