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Isolation and Characterization of Shewanella species from Hospital Effluence

Tapti Sengupta, Atanu Chakraborty, Trithankar Saha, Debopriya Nath, Parijat Das

Abstract


There is a growing concern about bacterial resistance to antimicrobial. Huge amount of antimicrobials are used in hospitals for the treatment of patients and disinfections. Untreated wastewater from hospitals, clinics and healthcare units contains nutrients and other components that aid the growth of microorganisms, especially bacteria. Shewanella species are opportunistic pathogens which cause hepatobiliary disease, peripheral vascular disease, with a chronic leg ulcer, and soft tissue infections in humans. Shewanella species represent a threat to public health due to its increasing resistance towards drug regime. The study was carried out to investigate the prevalence of Shewanella species in hospital effluents, its physiological and biochemical characteristics, and to determine the antibiotics susceptibility profile against selected antibiotics. Wastewater samples were collected from eight different hospitals, clinics and healthcare units around North 24 Parganas, West Bengal, and cultured on nutrient agar medium. The bacterial strains obtained from the wastewater samples were subjected to Gram-staining, confirmatory test, biochemical test, and antibiotics susceptibility tests, using the disc diffusion technique. A total of three Shewanella spp. were isolated based on their physiological and biochemical characteristics.

Keywords: antibiotics, biomedical wastes, microorganisms, species
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