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Gene Cloning

Prateek Sharma

Abstract


Gene cloning (DNA cloning) is a technique used for the generation of precise (clones) of a specific quality or DNA sequence utilizing genetic engineering techniques. The DNA containing the target gene(s) is cut into sections utilizing restriction enzymes. These sections are then embedded into cloning vectors, for example, bacterial plasmids or bacteriophages, which exchange the recombinant DNA to reasonable host cells, for example, the bacterium E. coli. On the other hand, complementary DNA is embedded into the vectors, or "naked" DNA pieces can be taken up directly by a host bacterium from its medium (this is less effective than vector exchange).

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