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An Evaluation of Lactate Dehydrogenase in the Lindane Exposed Fresh Water Fish, Channa punctatus

Aradhna Gupta

Abstract


The objective of this study was to investigate the changes in lactate dehydrogenase (LDH) activity and its isozyme profile in the lindane exposed fish, Channa punctatus, after 96h treatment with three subacute concentrations (0.025, 0.05, 0.1mg/l)). The results showed decreased activity of LDH in different organs of the fish; the values recorded were in following order: gills>brain>liver>muscle>heart>kidney. The maximum decrease was found in liver (22.53%) and minimum in muscle (12.74%). The kidney, heart, brain and gills registered 21.95, 20.06, 16.34 and 14.98% decrease, respectively, at highest concentration (0.1mg/l) of pesticide tested. The isozyme pattern of LDH showed presence of three isozyme activity bands in the control fish muscle, whose intensity decreased due to treatment of different concentrations of the pesticide. At maximum concentration of pesticide (0.1ppm) tested; the middle band of the isozyme got disappeared showing isozyme specific impact of the lindane in C. punctatus. The results indicated that lindane exposure caused perturbations in the energy metabolism via significant reduction in the activity of LDH in the freshwater fish muscle. The levels of LDH thus may be exploited as a potential indicator for biological monitoring of pesticide pollution in aquatic reservoirs.


Keywords


Lactate dehydrogenase, lindane, isoenzyme, Channa punctatus, muscle

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