Open Access Open Access  Restricted Access Subscription or Fee Access

Effects of Habb-e-Shifa a Formulation of Unani Medicine on Naloxone Precipitated Morphine Withdrawal Symptoms in Mice

Mujassam M., Sofi G., Shabir P., Wasim A.

Abstract


Habb-e-Shifa is a compound herbal formulation of Unani system of medicine claims to be useful in the patients addicted to opium and helps to improve the withdrawal syndrome. The study was carried out in male Swiss mice weighing 20–30 g. All the animals were divided into five groups of six animals in each and induced with administration of morphine s.c. for four days. Group I served as negative control (NC) and treated with 1 ml normal saline with morphine, Group II (HS A) and Group III (HS B) treated with Habb-e-Shifa extract (50 mg/kg and 100 mg/kg) on the day of four and Group IV (HS CA) and V (HS CB) treated with same dose daily twice concurrently with morphine. All the animals were received the last dose of Habb-e-Shifa extract with morphine two hours before naloxone injection on day four and were assessed for number of jumping in cylindrical jar immediately after the injection of naloxone over a period of 30 minutes. It was found that mean number of jumping in HS A, HS B, HS CA and HS CB was significantly decreased (p<0.05). All the groups of animals were compared with NC. Test drug Habb-e-Shifa produced significant degree of effect on morphine withdrawal syndrome and thus validated the Unani Medicine claim that Habb-e-Shifa is an important drug to be useful in the management of opioid withdrawal.

Keywords


Habb-e-Shifa, morphine withdrawal, naloxone challenge, opioid dependence, Naloxone

Full Text:

PDF

References


Costa E, Cheney DL, Racagni G. An analysis at synaptic level of the morphine action in striatum and N. accumbens: dopamine and acetylcholine interactions. Life Sci. (1975), 17(1):1–8.

Moroni F. Cheney DL, Costa E. The turnover rate of acetylcholine in brain nuclei of rats injected intraventricularly and intraseptally with alpha and beta-endorphin. Neuropharmacology, (1978), 17(3), 191–61.

Donna Flavo RN., Medical and psychosocial aspect of chronic illness and disability, III Ed. (2005); Jones and Bartlett Publishers, p; 206

Joseph T., Dipiro et al, Pharmacotherapy a pathophysiologic approach, 7th Ed. (2008), Mc Graw Hill, New York.

Heidbreder, C., 2005. Novel pharmacotherapeutic targets for the management of drug addiction. European Journal of Pharmacology, 526(1–3), pp.101–112.

Tripathi KD, Essential of Medical Pharmacology, 7nd Ed. Jaypee Brothers Medical Publishers (P) Ltd., (2013), p.483.

Shirazi IM, Risalah Afiyun (Urdu Translation by Bari A), Govt, of India, Ministry of Health and Family Welfare, Deptt. of Ayush, New Delhi, (2009), p; 43–45, 51–54, 63–86.

Rahman S, Khan RA, Kumar A. Experimental study of the morphine de-addiction properties of Delphinium denudatum Wall. BMC Complementary and Alternative Medicine (2002), 2(1), p.6.

Gomaa, A., Hashem, T., Mohamed, M. and Ashry, E., 2003. Matricaria chamomilla extract inhibits both development of morphine dependence and expression of abstinence syndrome in rats. Journal of pharmacological sciences, 92(1), pp.50–55.

Kumarnsit, E., Keawpradub, N., Vongvatcharanon, U., Sawangjaroen, K. and Govitrapong, P., 2005. Suppressive effects of dichloromethane fraction from the Areca catechu nut on naloxone-precipitated morphine withdrawal in mice. Fitoterapia, 76(6), pp.534–539.

Tajuddin M., Khan NA., Tajuddin, Afzal M. Analgesic, anticonvulsant and antipyretic effects of a Unani compound formulation Habb-e-Shifa., Indian Journal of Traditional Knowledge, Vol. 6(2), April 2007, pp.315–318.

Freireich EJ, et al. Quantitative Comparison of Toxicity of Anticancer Agents in Mouse, Rat, Dog, Monkey and Man. Cancer Chemotherapy reports, Europe PMC (1 May 1966), 50(4): 219–244.

Zarrindast, M.R. and Farzin, D., 1996. Nicotine attenuates naloxone-induced jumping behaviour in morphine-dependent mice. European Journal of Pharmacology, 298(1), pp.1–6.

Marshall, E.J., Edwards, G. and Taylor, C., 1994. Mortality in men with drinking problems: a 20‐year follow‐up. Addiction, 89(10), pp.1293–1298.

Mann, K., 2004. Pharmacotherapy of alcohol dependence. CNS Drugs, 18(8), pp.485–504.

Suh, J.J., Pettinati, H.M., Kampman, K.M. and O'Brien, C.P., 2006. The status of disulfiram: a half of a century later. Journal of Clinical Psychopharmacology, 26(3), pp.290–302.

Rekka, E.A., Kourounakis, A.P. and Kourounakis, P.N., 1996. Investigation of the effect of chamazulene on lipid peroxidation and free radical processes. Research communications in molecular pathology and pharmacology, 92(3), pp. 361–364.

Ertekin, V., Selimoğlu, M.A. and Altinkaynak, S., 2005. A combination of unusual presentations of Datura stramonium intoxication in a child: Rhabdomyolysis and fulminant hepatitis. Journal of Emergency Medicine, 28(2), pp.227–228.

Friedman, M., 2004. Analysis of biologically active compounds in potatoes (Solanum tuberosum), tomatoes (Lycopersicon esculentum), and jimson weed (Datura stramonium) seeds. Journal of Chromatography A, 1054(1–2), pp.143–155.

Rodrigo, G.J. and Rodrigo, C., 2002. The role of anticholinergics in acute asthma treatment: an evidence-based evaluation. Chest, 121(6), pp.1977–1987.

Maruyama Y., Takemori AE., the role of dopamine and norepinephrine in the naloxone-induced abstinence of morphine dependent mice. J. Pharmacol. Exp. Ther. (1973), 185(3):602–608.

Ho, I.K., Loh, H.H. and Way, E.L., 1973. Influence of 5, 6-dihydroxytryptamine on morphine tolerance and physical dependence. European Journal of Pharmacology, 21(3), pp.331–336.

Andrade, R., Vandermaelen, C.P. and Aghajanian, G.K., 1983. Morphine tolerance and dependence in the locus coeruleus: single cell studies in brain slices. European Journal of Pharmacology, 91(2–3), pp.161–169.


Refbacks

  • There are currently no refbacks.