Caffeine Prove as Neurostimulant by Enhancing the Acid and Alkaline Phosphatase Levels in Young Rat Liver and Young and Old Aged Wistar Rat Brain

Abstract

ABSTRACT
Acid phosphatase (ACP) and Alkaline phosphatase (ALP) specific activity was estimated for young (150–220 g) and old aged (320–520 g) wistar rats and for similar groups with caffeine (neurostimulant) treated whole brain and liver tissues. Rats were orally administered with caffeine 13 mg/kg body weight for 3 and 6 days. Brains of young and old rats showed significant increased ACP activity 34.21 and 22.836 nmol/min/mg of protein for 3 days caffeine treatment than 6 days treated 18.812 and 9.692 as compared to their respective control 3.874 and 7.133 nmol/min/mg, respectively. Young and old rat liver showed ACP specific activity 146.217, 108.952 and 85.222, 103.381 for 3 and 6 days, respectively for caffeine treatment as compared to respective control 92.030 and 100.621 nmol/min/mg, respectively. Young and old rat brains showed significant increased ALP specific activity 22.6467 and 18.01 nmol/min/mg of protein, respectively for 3 days caffeine treatment as compared to 6 days treated 14.576 and 10.236 as compared to their control 5.238 and 8.91 nmol/min/mg, respectively. ALP showed lower specific activity of 36.14 and 41.44 for 3 and 6 days, respectively in old rat liver caffeine treatment as compared to control 46.36 nmol/min/mg, while young liver showed significant elevated ALP activity of 82.741 and 44.48 for three and six days caffeine treatment, respectively as compared to control 30.995 nmol/min/mg of protein. Current results suggested that caffeine is significant positive stimulator for elevated ACP specific activity and ALP for young brain, old rat brain and young liver, however old liver did not respond to caffeine treatment on ALP higher activity, whereas old brain has got significant positive effect for both phosphatases that prove caffeine is neurostimulant that plays an important role in old brain cell function.

Keywords: acid phosphatase (ACP), alkaline phosphatase (ALP), caffeine, neurostimulant, specific activity

Cite this Article: Prabha M, Suneetha P, Shailabbh Aggrahari, Ramez Ahmed, Priya Prabhu, Rachita KR. Caffeine Prove as Neurostimulant by Enhancing the Acid and Alkaline Phosphatase Levels in Young Rat Liver and Young and Old Aged Wistar Rat Brain. International Journal of Animal Biotechnology and Applications. 2019; 5(1 & 2): 38–48p.