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Cost-Effective Procedure of Agarose Gel Recycling

Hamid Mustafa, Adeela Ajmal, Noor- ul- Ain, Muhammad Thair Khan

Abstract


Agarose gel electrophoresis is a commonly used technique in various areas of biotechnology. This pretentious but specific analytical procedure is used in research, biomedical, and forensic laboratories. Agarose gel recycling is a realistic approach for molecular genetic studies. Reuse of agarose gel for daily routine experiments to save money due to limited financial resources for research. This practice satisfies a need to control expenses. Recycled agarose can be used for the examination of genomic DNA, PCR-RFLP analysis, or other applications in which no further manipulation of the DNA is necessary. Here, we report a simple but improved recycling method, which is comparatively similar to fresh agarose gel.

Keywords


agarose, DNA, RFLP, recycle

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References


Gimdnez C. Caracterfsticas Gen6ticas y Moleculares del Variante somaclonal de Banano (CIEN BTA-03) Asociadas al Mecanismo de Resistencia a la Sigatoka Amarilla y a su Estabilidad Gen6tica. Universidad Central de Venezuela. Facultad de Ciencias. Post grado de Botfinica; 1998. 144p.

Gustavo P., Carlos G., Eva D.G. Recycling agarose. Plant Mol Biol Rep. 2000; 18: 47–9p.

James P.H., Scott M.G., Philippe M., et al. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in cambridge and belclare sheep (ovis aries). Biol Reprod. 2004; 70: 900–9p.

Montgomery G.W., Sise J.A. Extraction of DNA from sheep white blood cells. NZ J Agric Res. 1990; 33: 437–41p.


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