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Intracytoplasmic Sperm Injection (ICSI): A Review

Sreeremya Sasi


Intracytoplasmic sperm injection (ICSI) is a recently developing technique in the field of developmental biology. The rationale behind artificial insemination is increasing the gamete density at the site of fertilization. Since many centuries diverse pioneers added to the historical backdrop of manual sperm injection, not only in humans but even more pronounced in farm animals. The primary reason for using this technique in farm animals is to speed up the rate of genetic improvement by increasing the productivity of food producing animals. This review paper provides an insight about the prominence of ICSI technique.

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B. Wang, H. Baldassarre, J. Pierson, F. Cote, K.M. Rao, C.N. Karatzas. The in vitro and in vivo development of goat embryos produced by intracytoplasmic sperm injection using tail-cut spermatozoa, Zygote. 2003; 11: 219–27p.

N.Y. Zhang, H.X. Sun, Y.L. Hu, B. Wang, Z.P. Xu. Early rescue intracytoplasmic sperm injection: safe for complete IVF failure, Zhonghuanankexue. 2010; 16: 158–60p.

M.H. Sekhavati, F. Shadanloo, M.S. Hosseini, M. Tahmoorespur, M.R. Nasiri, M. Hajian, M.H. Nasr-Esfahani. Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment, Cell Reprog. 2012; 14: 295–304p.

H. Yanagimachi. Intracytoplasmic sperm injection experiments using the mouse as a model, Hum. Reprod. 1998; 13: 87–98p.

H. Katayose, Y. Maeda, Y. Kimura, K. Suzuki, K. Yanagida, et al. Successful ICSI by using piezo micromanipulator in bovine oocytes, J Mammal Ova Res. 1997; 14: S30p.

Z.P. Nagy, H. Joris, J. Liu, C. Staessen, P. Devroey, A.C. Van Steirteghem. Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes, Hum Reprod. 1993; 8: 2180–4p.

J.H. Moon, W.Y. Son, S. Henderson, A. Mahfoudh, M. Dahan, H. Holzer. Spindle examination in unfertilized eggs using the polarization microscope can assist rescue ICSI, Reprod Biomed. 2013; 26: 280–5p.

R. Marci, F. Murisier, G. Lo Monte, I. Soave, A. Chanson, F. Urner, M. Germond. Clinical outcome after IMSI procedure in an unselected infertile population: a pilot study, Reprod Health. 2013; 10: 16–20p.

R.D. Leandri, A. Gachet, J. Pfeffer, C. Celebi, N. Rives, F. Carre-Pigeon, O. Kulski, V. Mitchell, J. Parinaud. Is intracytoplasmic morphologically selected sperm injection (IMSI) beneficial in the first ART cycle? a multicentric randomized controlled trial, Andrology. 2013; 1: 692–7p.

S. Cao, X. Wu, C. Zhao, L. Zhou, J. Zhang, X. Ling. Determining the need for rescue intracytoplasmic sperm injection in partial fertilisation failure during a conventional IVF cycle, Andrologia. 2016; 48: 1138–44p.



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