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In-Vitro and In-Vivo Selection of Efficient Growth Promoting Fluorescent Pseudomonas Isolated from Bamboo Rhizosphere

Solomon Das, Y. P. Singh, Y. K. Negi, P. C. Shrivastav

Abstract


Bamboo is one of the important plant species for sustainability in both forestry and agriculture sector. Among bamboos, Dendrocalamus strictus is widely present in Indian sub-continent. Though bamboo having adventitious as well as shallow root system, it is one of the fastest growing plant species. This may be due to its dependency on several symbiotic soil microbes which may be in return providing nutrients and water for its fast growth. As Indian soil is deficient in available phosphorus, exploring the possibility of finding growth promoting bacteria, such as fluorescent pseudomonas from such niche is a matter of research. The study explored the rhizosphers of D. strictus growing in three geographical regions. The result concluded with finding fluorescent pseudomonas which efficient in P solubilising from P deficient soil of Chiriapur region. It seems that soil having moisture and nutrient stress environment allowed the rhizospheric bacteria to evolve to be efficient, especially P solubilisers, in relation to plant.

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Forest Survey of India. 2011. State of forest report. Dehra Dun, FSI (www.fsi.nic.in). 210p.

T.A. Thomas, R.K. Arora, R. Singh. Genetic resources of bamboo in India: Their diversity, utilization and socio-economic role. In: Proceedings of the International Conference, Honghzou, China, October 6–14, 1985.

S. Biswas. Studies on bamboo distribution in North-eastern region of India, Ind. For. 1988; 114: 514–7p.

T. Muthukumar, K. Udaiyan. Growth of nursery-grown bamboo inoculated with abruscular mycorrhizal fungi and plant growth promoting rhizobacteria in two tropical soil types with and without fertilizer application, New Forests. 2006; 31: 469–85p.

P.N. Deogun. The silviculture and management of the bamboo Dendrocalamus strictus Nees. 1937; 2(4): 173p.

S. Das, Y.P. Singh, Y.K. Negi, P.C. Shrivastav. Genetic variability in different growth forms of Dendrocalamus strictus: Deogun Revisited, New Zealand J Forest Sci. (Accepted) DOI 10.1186/s40490-017-0104-4.

J.W. Kloepper, M.N. Schroth. Relationship of in vitro antibiosis of plant growth promoting rhizobacteria to plant growth and the displacement of root microflora, Phytopathology. 1981; 71: 1020–4p.

R.N. Chakraborty, H.N. Patel, S.B. Desai. Isolation and partial characterization of catechol-type siderophore from Pseudomonas stutzeri RC-7, Curr Microbiol. 1990; 20: 283–6p.

H.G. Didick, Siswanto, Y. Sugiarto. Bioactivation of poorly soluble phosphate rocks with a phosphorus solubilizing fungus, Soil Sci Soc Am J. 2000; 64: 927–32p.

F. Ahmad, I. Ahmad, M.S. Khan. Indole acetic acid production by the indigenous isolates of azotobacter and fluorescent in the presence and absence of tryptophan, Turk J Biol. 2005; 29: 29–34p.

H. Fankem, D. Nwaga, A. Deubel, L. Dieng, W. Merbach, F. F.X. Etoa. Occurence and functioning of phosphate solubilising microorganisms from oil palm tree rhizosphere in cameroon. Afr J Biotechnol. 2006; 5(24): 2450–60p.

W.A. Gloria, A.W. Hoadley. Fluorescent pseudomonads capable of growth at 41ºC but distinct from Pseudomonas aerugenosa, J Clin Microbiol. 1976; 4: 443–9p.

C.S. Nautiyal. An efficient microbiological growth medium for screening phosphate solubilising microorganisms, FEMS Microbiol Lett. 1999; 170(1): 265–70p.

T.C. Baruah. Effect of soil water regimes on plant resistance to water transport and root growth of some rice varieties, M.Sc. (Ag.) Thesis. submitted to G.B. Pant University of Agriculture and Technology, Pantnagar, 1975, 89p.

P. Illumer, F. Schinner. Solubilization of inorganic calcium phosphate-solubilization mechanisms, Soil Biol Biochem. 1995; 27: 257–63p.

A.E. Richardson, P.A. Hadobas, J.E. Hayes, J.E. O'Hara, R.J. Simpson. Utilization of phosphorus by pasture plants supplied with myoinositol hexaphosphate is enhanced by the presence of soil microorganisms, Plant Soil. 2001; 229: 47–56p.

C.S. Nautiyal, S. Bhaduria, P. Kumar, H. Lal, R. Mondal, D. Verma. Stress induced phosphate solubilization in bacteria isolated from alkaline soils, FEMS Microbial Lett. 2000; 182: 291–6p.

A.E. Carrillo, C.Y. Li, T. Bashan. Increased acidification in the rhizosphere of cactus seedlings induced by Azospirillum brasilense, Naturwissenschaften. 2002; 89: 428–32p.

H. Rodriguez, T. Gonzalez, I. Goire, Y. Bashan. Gluconic acid production and phosphate solubilization by the plant growth promoting bacterium Azospirillum spp. Naturwissenschaften. 2002; 91: 552–5p.

A.C. Gaur. Phosphate Solubilizing Microorganisms as Bio-fertilizers. Omega Scientific Publication: New Delhi; 176p.

D. Thakur, M. Kaur, V. Shyam. Optimization of best cultural conditions for high production of phosphate solubilizing activity by fluorescent pseudomonas isolated from normal and replant sites of apple and pear, Bioscan. 2014; 9(1): 143–50p.

S.K. Jena, C.R. Chandi. Optimization of culture conditions of phosphate solubilizing activity of bacterial sp. isolated from Similipal biosphere reserve in solid-state cultivation by response surface methodology, Int J Curr Microbiol Appl Sci. 2013; 2(5): 47–59p.

H.A. Louw, D.M. Webley. A study of soil bacteria dissolving certain phosphate fertilizers and related compounds, J Appl Bacteriol. 1959; 22: 227–33.

A.C. Das. Utilization of insoluble phosphates by soil fungi, J Indian Soc Soil Sci. 1963; 11: 203–7p.

K.P. Ostwal, V.P. Bhide. Solubilization of tricalcicum phosphate by soil Pseudomonas, Indian J Exp Biol. 1972; 10: 153–64p.

H. Katznelson, E. Peterson, J.W. Rovatt. Phosphate dissolving microoganisms on seed and in the root zone of plants, Can J Bot. 1962; 40: 1181–6p.

M.C. Bardiya, A.C. Gaur. Isolation and screening of microorganisms dissolving low grade rock phosphate, Folia Microbiol. 1974; 19: 386–9p.

A.H. Goldstein, S.T. Liu. Molecular cloning and regulation of a mineral phosphate solubilizing gene from Erwinia herbicola, BioTechnology. 1987; 5: 72–4p.

N.S. Darmwall, R.B. Singh, R. Rai. Isolation of phosphate solubilizers from different sources, Curr Sci. 1989; 58: 570–1p.

P. Illmer, F. Schinner. Solubilization of inorganic phosphates by microorganisms isolated from forest soil, Soil Biol Biochem. 1992; 24: 389–95p.

R. Deepa. Mineral phosphate solubilization by fluorescent pseudomonads, M.Sc. (Agri.) Thesis. University of Agricultural Sciences, Dharwad.

K. Suneesh. Biodiversity of fluorescent pseudomonads in soils of moist-decidous forests of Western Ghats of Uttara Kannada district, M.Sc. (Agri.) Thesis. University of Agricultural Sciences, Dharwad, 2004, 134p.

D. Kim, G.A. Jordan, G.A. McDonald. Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity, Biol Fert Soils. 1997; 26(2): 79–87p.

C.D. Di Simine, J.A. Sayer, G.M. Gadd. Solubilization of zinc phosphate by a strain of Pseudomonas fluorescens isolated from forest soil, Biol Fert Soils. 1998; 28: 87–94p.

P.U. Krishnaraj. Genetic characterization of mineral phosphate solubilization in Pseudomonas spp., Ph.D. Thesis. Indian Agricultural Research Institute, New Delhi, 180p.

A.H. Goldstein. Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphates by gram-negative bacteria, In: Phosphate in Microorganisms: Cellular and Molecular Biology. Washington, DC, ASM Press; 1994, 197–203p.

D.H. Goenadi, Siswanto, Y. Sugiarto. Bioactivation of poorly soluble phosphate rocks with phosphorus solubilizing fungus, Soil Sci Soc Am J. 2000; 64: 927–32p.

B.S. Kundu, R. Gera. Host specificity of phosphate solubilizing bacteria, Indian J Microbiol. 2002; 42: 19–21p.

K.P. Pallavi, P.C. Gupta. Effect of different carbon and nitrogen sources on solubilization of insoluble inorganic phosphate by psychrotolerant bacterial strains, Bioscan. 2013; 8(4): 1299–302p.

D. Thakur, M. Kaur, V. Shyam. Optimization of best cultural conditions for high production of phosphate solubilizing activity by fluorescent pseudomonas isolated from normal and replant sites of apple and pear, Bioscan. 2014; 9(1): 143–50p.

H. Fankem, D. Nwaga, A. Deubel, L Dieng, W. Merbach, F.X. Etoa. Occurence and functioning of phosphate solubilising microorganisms from oil palm tree rhizosphere in cameroon, Afr J Biotechnol. 2006; 5(24): 2450–60p.

P. Trivedi, T. Sa. Pseudomonas corrugate (NRRL B-30409) mutants increased phosphate solubilization, organic acid production and plant growth at lower temperatures, Curr Microbiol. 2008; 56: 140–4p.

P. Vyas, P. Rahi, A. Gulati. Stress tolerance and genetic variability of phosphate solubilizing fluorescent pseudomonas from the cold deserts of the trans-Himalayas, Microbial Ecol. 2009; 58: 425–34p.

S. Rawat. Bamboo-endomycorrhiza: Ecology, growth and macroproliferation, Ph. D. Thesis. Forest Research Institute (Deemed) University, Dehradun, 2005, 200p.

B.R. Glick. The enhancement of plant growth by free-living bacteria, Can J Microbiol. 1995; 41: 109–17p.


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