Open Access Open Access  Restricted Access Subscription or Fee Access

Development of In-Vitro Xylanase Digestion (IVXD) Strategy Using Agriculture Wastes for the Production of Exopolysaccharide Through Solid-State Fermentation Using Lactobacillus plantarum MTCC 2621

Pankit Gautam, Barkha Singhal

Abstract


In this research work, the study of exopolysaccharides, structure and amount of the exopolysaccharides (EPS) and these are produced by Lactic acid bacteria (Lactobacillus plantarum and Bacillus licheniformis), plants, and fungus. Lactic acid bacteria are gram-positive and non-sporulating that ferment various carbohydrates like as lactate and acetate and various advantages of this, like as EPSs are economically important because exopolysaccharides have many applications in the food industries, pharmaceutical industries, and other industries and eps production, enzyme production, organic acid production by the solid-state fermentation. Solid-state fermentation (SSF) defines the growth of microorganism without free-flowing aqueous phase. In the solid-state fermentation, wheat bran and pretreatment substrate were used like as rice straw (alkali pretreatment) and acid (pretreatment). Almost known microbial enzymes can be produced by the solid-state fermentation techniques. Enzymes of industrial importance, like cellulose, protease, pectinases, xylanases, amylase, glucoamylases, etc. used the solid-state fermentation. In this research work, the production of the exopolysaccharides (EPS) and to check the xylanase activity from the different-different methods these are phenol-sulphuric acid, Lowry method and di-nitro salicylic (DNS) acid method.

Full Text:

PDF

References


H. Kumon, K. Tomoshika, T. Matunaga, M. Ogawa, H.A. Ohmori. Sandwich cup method for the penetration assay of antimicrobial agents through Pseudomonas exopolysaccharides, Microbiol Immunol. 1994; 38: 615–9p.

S.V. Dilna, H. Surya, R.G. Aswathy, K.K. Varsha, D.N. Sakthikumar, et al. Characterization of an exopolysaccharide with potential healthbenefit properties from a probiotic Lactobacillus plantarum RJF4, LWT Food Sci Technol. 2015; 64: 1179–86p.

E. Cannel, M. Moo-Young. Solid state fermentation systems, Proc Biochem. 1980; 15: 2–7p.

M. Raimbault. General and microbiological aspects of solid substrate fermentation, Electron J Biotechnol. 1998; 1(3): 1–15p.

C.W. Hesseltine. Solid statefermentation, Biotechnol Bioeng. 1972; 14: 517–32p.

K.E. Aidoo, R. Hendry, et al. Estimation of fungal growth in solid state fermentation, Eur J Appl Microbiol Biotechnol. 1981; 12: 6–9p.

M.E. Flores, R. Pérez, C. Huitrón. β-Xylosidase and xylanase characterization and production by streptomyces sp. CH-M-1035, Lett Appl Microbiol. 1997; 24(5): 410–6p.

M. Bataillon, A.P. Nunes Cardinali, F. Duchiron. Production of xylanases from a newly isolated alkalophilic thermophilic Bacillus sp, Biotechnol Lett. 1998; 20(11): 1067–71p.

Archana, T. Satyanarayana. Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation, Enzyme Microb Technol. 21(1): 12–17, 19p.

Salminen et al. 1999; Holzapfel & Schillinger, 2002

M. De Vries, E. Vaughan, M. Kleerebezem, W. De Vos. Lactobacillus Plantarum—survival, functional and potential probiotic, Int Dairy J. 2006; 16: 1018–28p.

E. Giraud, A. Champailler, R. Raimbult. Degradation of raw starch by a wild amylolytic strain of Lactobacillus platarum, Appl Environ Microbiol. 1994; 60: 4319–23p.

S. Liu. A simple method to generate chromosomal mutations in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products, Appl Biochem Biotechnol. 2006; 131: 854–63p.

B.C. Saha, M.A. Cotta. Lime pretreatment, enzymatic saccharification and fermentation of rice hulls to ethanol, Biomass Bioenergy. 2008; 32: 971–7p.

M. Kleerebezem, J. Boekhoerst, R. Kranenburk, et al. Complete Genome Sequence of Lactobacillus plantarum WCFS1, PNAS. 2003; 100: 1990–5p.




DOI: https://doi.org/10.37628/ijcbb.v3i2.244

Refbacks

  • There are currently no refbacks.