Purification and Characterizations of Antimicrobial Protein from Calotropis procera
Calotropis procera latex contain cardioids such as calotropin, calotoxin, uscherin, uscchardin, glycoside calotropaginin, choline, o-pyrocatechuric acid, benzoyllineolone, benzoylisoloneolane, uzariganinand syriogenis proteins such as osmotin, calotropin, etc.
Osmotropin and calotropin shows the antimicrobial activity against microbe. Here, we were isolated a novel protein from latex using ionexchange and gel filter Ttration chromatography that shows the antimicrobial activity. Antimicrobial activity assay done against bacterial and fungi. the bacterial and fungi strains are collected from Gautam Buddha university Greater Noida (UP). E. Coli, S. enterica, Vibro sp. P. aeruginosa, Klebsiella pneumoniae, Fusarium sp., and Saccharomyces sp. microbe were used for antimicrobial assay at different pH, temperature, and concentration. Protein being crystalized for structural and functional application.
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