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Investigation for the Colistin Resistance Genes MCR-1 and MCR-2 in E. Coli Isolates from Urinary Tract Infected Patients

K. Jagathy, S. Malathi

Abstract


Background and Objective: The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from Urinary tract infected patients in Clinical Microbiology Laboratory of Saveetha Medical College and Hospital during the period of (Nov 2019-Feb 2020). Methods: The present study is to check the prevalence, demonstrate the virulence factors and study the antibiotic susceptibility pattern of Escherichia coli in our clinical settings. Phenotypic colistin resistance was screened by the antibiotic broth microdilution method. PCR was done to identify mcr-1 and mcr-2 genes. Results: Our study revealed for the first time, a surprisingly high percentage (20%) of colistin-resistant E. coli carrying chromosomal mcr-1 and mcr-2 genes. Full gene sequencing confirmed that 11 strains encoded mcr-1. The other 9 colistin-resistant isolates were found to have mcr-2 genes using specific primers. We observed an unexpectedly high prevalence of mcr-1 than mcr-2 in patients attending a tertiary care hospital by PCR with an estimation of 9.0% (95% confidence interval). The mcr-1 and mcr-2 genes were detected predominantly in Escherichia coli. Conclusion: Our results are valuable in understanding the mechanism underlying the increasing prevalence of colistin-resistant bacteria within a community.

Keywords: Colistin; resistance, Escherichia coli, Polymerase chain reaction, Microdilution method.

Cite this Article: K. Jagathy, S. Malathi. Investigation for the Colistin Resistance Genes MCR-1 and MCR-2 in E. Coli Isolates from Urinary Tract Infected Patients. International Journal of Biochemistry and Biomolecules. 2020; 6(3): 24–33p.



Keywords


Colistin, resistance, Escherichia coli, polymerase chain reaction, microdilution method

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