Production and Characterization of Thermostable Amylase from Bacillus korlensis Isolated from Unkeshwar Hot Spring, Nanded

Abstract

ABSTRACT
Microorganisms are ubiquitous and dwell in both moderate and extreme ecological habitats like hot springs, alkaline lakes, solar saltern, glacier, etc. Thermophiles isolated from various hot springs are the sources of industrially important thermozymes and other bioactive compounds with scientific and commercial interests. To this scenario, we selected the Unkeshwar hot spring from Maharashtra and reported it’s scrupulous cultivable bacterial diversity in our previous publications. As per our knowledge and available literature, very few researchers have performed scientific research on Unkeshwar hot spring. Bacillus korlensis was one of the efficient thermostable amylase producers from our collection of isolates from Unkeshwar hot spring. It was Gram-stain positive, spore former, motile and rod shaped bacterium. The colonies were pale brown, circular with shiny glistering and granular surface. It has shown acid production from arabinose, cellobiose, fructose, galactose, glucose, glycerol, lactose, mannitol, maltose, ribose, salicin, and sucrose. It was catalase and oxidase positive. Optimum temperature for growth of Bacillus korlensis was recorded 50oC. Beside amylase production, it has also shown thermostable protease (caseinase) production ability. The production of thermostable amylase from Bacillus korlensis was recorded 230 U/mL. Amylase from Bacillus korlensis has shown optimum amylolytic activity at temperature 65oC and pH 7. Catalytic activity of partially purified amylase from Bacillus korlensis was enhanced in presence of 1 Mm MgCl2, CaCl2 and ZnCl2. In conclusion, Bacillus korlensis amylase can be used in various aspects of enzyme biotechnology like industrial use and molecular studies of biomolecules.

Cite this Article: Anupama Prabhakarrao Pathak, Mukundraj Govindrao Rathod. Production and Characterization of Thermostable Amylase from Bacillus korlensis Isolated from Unkeshwar Hot Spring, Nanded. International Journal of Biochemistry and Biomolecules. 2019; 5(2): 8–11p.

Keywords: Thermophiles, Thermozymes, Hot spring, Catalytic activity, Metal cations