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Effects on Growth patterns of Marine Microalgae and Macroalgae in Different Media to Increase the Omega-3 Fatty Acid Production

Vishwanatha H N, Shivakumar R, Samita Maitra

Abstract


Eicosa-Pentaenoic Acid, Docosa-Hexaenoic Acid and Alpha- Linolenic Acid are used as dietary supplement. In the present investigation Pavlova lutheri (marine microalgae) and Azolla filiculoides (fresh water macroalgae) are used to study on different stress conditions for the accumulation of lipids using microalgae and macroalgae. The effect of various parameters such as light intensity, natural sea water, artificial sea water and nutrient deprived in HESNW (High Enrichment Salt Nutrient Water) media are studied. Under sodium deprived condition, Pavlova lutheri showed a significant decrease in growth rate and increase in lipid accumulation, when compared to the growth in normal HESNW media. Azolla filiculoides also showed a significant decrease in growth rate and increase in lipid accumulation for magnesium andpotassium deprived media. High lipid accumulation was found in potassium stress compared to magnesium stress conditions.Overall results found that Azolla filiculoidesalgae showed a better lipid production at optimum conditions.

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. H. Yassin, EI-Kassas. Growth and fatty acid profile of the marine microalga Picochlorum sp. grown under nutrient stress conditions, Egyptian J Aqu Res. 2013; 39: 233-239p.

. M. Óscar, CN. Juan, RT. Dougla. Long-chain polyunsaturated fatty acids in fish: recent advances on desaturases and elongases involved in their biosynthesis. Universidad Autónoma de Nuevo León, Monterrey, México. 2011;11: 257-283p.

. AT. Catalina, KYL. David. Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production. Microbial Cell Factories. 2012;11: 1-10p.

. N. Jagannathan, K. Amutha, N. Anand. Production of Omega-3 fatty acids in Cymbellasp, Adv Biores. 2010; 1(2): 40-45p.

. WR. Barclay, KM. Meager, JR. Abril. Heterotrophic production of long chain omega-3 fatty acids utilizing algae and algae-like microorganisms, J Phyco.1994; 6: 123-129p.

. PJ. Ashton, RD. Walmsley. The taxonomy and distribution of Azolla species in southern Africa, Bot J Linn Soc. 1984; 89:239-247p.

. G. Bougaran, C. Rouxel, N. Dubois. Enhancement of neutral lipid productivity in the microalga Isochrysisaffinis Galbana (T-Iso) by a mutation-selection procedure. Biotechnol Bioengg. 2012; 10: 2737-2745p.

. RM. Karthryn. Fish and shellfish as dietary sources of methylmercury and the Omega-3 fatty acids, eicosahexaenoic acid and docosahexaenoic acid: risks and benefits. Environ Res.2004; 95: 414-428p.

. BL. Oliveira, H. Hanh. Ultrastructure and cytochemistry of Dunaliellatertiolecta Butcher and Pavlova lutheri (Droop) Green grown on three different sources of organic nitrogen. New Phyto. 1989; 113: 481-490p.

. AM. Dulce, C. Luisa. Alternative sources of n-3 long-chain polyunsaturated fatty acids in marine microalgae. Marine Drugs. 2013; 11: 2259-2281p.

. E. Ponis, G.Parisi, R. Robert. Production, preservation and use as food for Crassostreagigas larvae. Aquacultre. 2008; l 282: 97-103p.

. P. Brouwer, A. Bräutigam, C. Külahoglu, A. Tazelaar, S. Kurz, K. Nierop, WA. Van der, A. Weber, H. Schluepmann. Azolla domestication towards a bio based economy?. New Phytol. 2014; 202: 1069-1082p.

. R. Cherad, JA. Onwudili, U. Ekpo, PT. Williams, AR. Lea-Langton, M. Carmargo-Valero, AB. Ross. Macoalgae supercritical water gasification combined with nutrient recycling for microalgae cultivation, Environ Prog Sustain Energy. 2013; 32: 902-909p.

. G. Adarsha, EA. Reinu, JB. Colin, P. Munish. Omega-3 fatty acids production from enzyme saccharified hemp hydrolysate using a novel marine thraustochytrid strain. Bioresour Technol. 2015; 184: 373-378p.

. RP. Anex, A. Aden, FK. Kazi, J. Fortman, RM. Swanson, M. Wright, JA. Satrio, RC. Brown, DE. Daugaard, A. Platon. Techno-economic comparison of biomass-to-transportation fuels via pyrolysis, gasification, Biochemical pathways. Fuel. 2010;89: 29-35p.

. KV. John, A. Graemw. Fatty acids from microalgae of the genus pavlova, Phytochemistry. 1991; 30: 1855-1859p.

. E. Martin. Docosahexaenoic acid production by the marine algae Crypthecodiniumcohnii. Rec Res Dev Microbiol. 2003; 2: 219-232p.

. RB. Malcolm. Nutritional value and uses of microalgae in aquaculture. CSIRO Marine Res. 2002; 58: 281-294p.

. SC. Michael, AL. Edward. A test of assumptions and predictions of recent miroalgal growth models with marine phytoplankter Pavlova lutheri. limnoloceanogr. 1990; 35(5): 583-596p.

. Y. Michael, S. Roleda, P. Slocombe. Effect of temperature and nutrient regimes on biomass and lipid production by six oleaginous microalgae in batch culture employing a two- phase cultivation strategy. Bioresour Technol. 2013; 129:439-449p.

. E. Ponis, G. Parisi, R. Robert. Effect of the culture system and culture technique on biochemical characteristics of Pavlova lutheri and its nutritional value for Crassostreagigas larvae. Aqu Nutri. 2006; 12: 322-329p.

. A. Liua, A. Meireles, GF. Catarina, M. Xavier. Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalgae Pavlova lutheri following random mutagenesis. Biotechnol Bioengg. 2003; 81(1): 50-55p.

. S. Lina, R. Lujing, Z. Xiaoyan. Differential effects of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp. Biores Tech. 2014; 15: 199-206p.

. PM. Del, S. Sanchez,V. Domenico. Culture of Pavlova lutheri (Droop) Green (Prymnesiophyta) in diluted wasrewater. J Appl Phycol. 1994; 6: 285-288p.

. L. Joon-Baek, K. Bo-Young. Growth characteristics of five microalgal species isolated from jeju island and four microalgae stock strains in hatchery. Algae. 2002; 17(2): 117-125p.

. K. Sumeet, SM. Dongre. Effect of environmental parameters enhancing the micro algal lipid as a sustainable energy source for biodiesel production-A Review. Int J Bio Pharma Res. 2014; 4: 327-335p.

. MU. Syed, AA. Shah. Enhancement of lipid content in isochrysisgalbana and pavlovalutheri using palm oil mill effluent as an alternative medium. Chem Engg Transaction. 2014; 37: 733-738p.

. LG. Xueping, L. Xiaoting. Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310. Bioresource Techn. 2015; 184: 139-147p.

. E. Ponis, I. Probert, B. Veron, M. Mathieu, R. Robert. New microalgae for the pacific oyster crassostreagigas larvae. Aquaculture. 2006;253: 618-627p.

. D. Malgorzata, P. Alina. Chromatographic methods in the separation of long-chain mono- and polyunsaturated fatty acids. J Chem. 2015: 1-20p


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